EMDB EMD-2248

Single particle reconstruction
43.1Å resolution

negative stain single-particle reconstruction of conformation I of the Ltn1 E3 ubiquitin Ligase

Map released:
Last modified:
Overview of EMD-2248
Source organism: Saccharomyces cerevisiae [4932]
Primary publication:
Single-particle EM reveals extensive conformational variability of the Ltn1 E3 ligase.
Lyumkis D, Doamekpor SK, Bengtson MH, Lee JW, Toro TB, Petroski MD, Lima CD, Potter CS, Carragher B, Joazeiro CA
PROC.NAT.ACAD.SCI.USA 110 1702-1707 (2013)
PMID: 23319619

Function and Biology Details

Sample name: snapshot I of Ltn1 conformer
Protein: Ltn1

Experimental Information Details

Resolution: 43.1Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: Spider, Appion
Microscope: FEI TECNAI F20
Detector: GATAN ULTRASCAN 4000 (4k x 4k)