EMD-2242 Experiments and Validation

The Cryo-EM structure of Arabis mosaic virus

Single particle reconstruction
Overview of EMD-2242
Sample name: Purified ArMV particle containing its RNA
Organism: Arabis mosaic virus

Map parameters

Recommended contour level: 50.30 (author)
Number of grid points: 281 × 281 × 281
Voxel size: 1.49 × 1.49 × 1.49 Å
Minimum density: 0.00
Maximum density: 100.00
Average density: 38.964
Standard deviation: 7.542

Sample information

Sample name: Purified ArMV particle containing its RNA
Virus: Arabis mosaic virus

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: BOXER, CTFFIND3, CTFMIX, AUTO3DEM
Number of particles: 7009
CTF correction: Each particle

Imaging

Session
Microscope model: JEOL 2200FS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 18.0
Energy filter: omega
Energy window (eV): 0.00
Nominal CS (mm): 2.00
Nominal magnification: 50000.0
Defocus max (nm): 2,500.00
Defocus min (nm): 1,200.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 400
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 2.00
Buffer: 15 mM sodium phosphate and 5 mM potassium phosphate pH 7.0
Specimen support: Quantifoil R 2/2 grids (Quantifoil Micro Tools GmbH, Jena, Germany)
Vitrification
Apparatus: GATAN CRYOPLUNGE 3
Cryogen: ETHANE
Humidity: 98
Protocol: blotted for 1s and then flash frozen in liquid ethane