EMD-2240 Experiments and Validation

3D structure of myosin filaments isolated from human heart muscles by negative stain electron microscopy and single particle image analysis.

Subtomogram averaging
Overview of EMD-2240
Sample name: 3D map of human cardiac myosin filament
Organism: Homo sapiens

Map parameters

Recommended contour level: 1.50 (author)
Number of grid points: 160 × 160 × 160
Voxel size: 5.94 × 5.94 × 5.94 Å
Minimum density: -8.243
Maximum density: 12.488
Average density: 0.043
Standard deviation: 0.776

Sample information

Sample name: 3D map of human cardiac myosin filament
Protein: myosin filament

Validation information

Experimental information


Image processing

Software: imagic
Reconstruction protocol: single particle analysis


Initial atomic model: 1myo
Fitting software: pymol and veda
Fitting protocol: rigid body
Refinement space: REAL
Details: Protocol: rigid body


Microscope model: FEI TECNAI 20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Nominal magnification: 29000.0
Holder model: OTHER
Number of images: 237
Sampling interval (μm): 17.0