EMD-2240 Experiments and Validation

3D structure of myosin filaments isolated from human heart muscles by negative stain electron microscopy and single particle image analysis.

Subtomogram averaging
Overview of EMD-2240
Sample name: 3D map of human cardiac myosin filament
Organism: Homo sapiens

Map parameters

Minimum density: -8.243
Maximum density: 12.488
Average density: 0.043
Standard deviation: 0.776
Recommended contour level: 1.50 (author)

Sample information

Sample name: 3D map of human cardiac myosin filament
Protein: myosin filament

Validation information

Experimental information

 

Image processing

Software: imagic
Reconstruction protocol: single particle analysis

Fitting

Initial atomic model: 1myo
Fitting software: pymol and veda
Fitting protocol: rigid body
Refinement space: REAL
Details: Protocol: rigid body

Imaging

Session
Microscope model: FEI TECNAI 20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Nominal magnification: 29000.0
Holder model: OTHER
Acquisition
Number of images: 237
Sampling interval (μm): 17.0