EMD-2238

Single-particle
7.5 Å
EMD-2238
3D View Gallery
Deposition: 05/12/2012
Map released: 16/01/2013
Last modified: 27/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2238
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4v8l summary report (pdf.gz) Map and 4v8l FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM Structure of the Mycobacterial Fatty Acid Synthase

EMD-2238

Single-particle
7.5 Å
EMD-2238
3D View Gallery
Deposition: 05/12/2012
Map released: 16/01/2013
Last modified: 27/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1.65 mg/mL
Buffer
pH: 7.2
Details: 100mM potassium phosphate buffer pH 7.2, 165 mM NaCl, 2mM EDTA, 2mM DTT
Grid
Details: Quantifoil R2/1 200 mesh copper grids
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.5 µm - 5.0 µm
Nominal magnification: 59000.0
Calibrated magnification: 100000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Data were collected using the automated image acquisition software FEI EPU.
Temperature
Average: 79 K
Image Recording [1]
Detector category: CCD
Detector model: FEI FALCON I (4k x 4k)
Number of real images: 1556
Average electron dose per image: 20 e/Å2
Bits per pixel: 16.0
Details: Data were collected using the automated image acquisition software FEI EPU.
Image processing [1]
Final reconstruction
Resolution: 7.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 106884
Algorithm: OTHER
⌯ Applied Symmetry
Point group: D3
Software [1]
Name Version Details
Imagic-5, Spider - -
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of the Mycobacterial Fatty Acid Synthase
Details: ::::EMDATABANK.org::::EMD-2238::::
⬡ Geometry
X Y Z
Dimensions 160 160 160
Origin 0 0 0
Spacing 160 160 160
Voxel size 2.45 Å 2.45 Å 2.45 Å
Contour list
Primary Level Source
True 2.7 AUTHOR