EMD-2235 Experiments and Validation

Electron cryo-microscopy of a head-tailed virus infecting extremely halophilic archaea

Single particle reconstruction
Overview of EMD-2235
Sample name: archaeal virus HSTV-2
Organism: HSTV-2
Related EM entry by publication: EMD-2234

Map parameters

Recommended contour level: -700.00 (emdb)
Number of grid points: 451 × 451 × 451
Voxel size: 2.26 × 2.26 × 2.26 Å
Minimum density: -2,266.00
Maximum density: 2,508.00
Average density: -916.08
Standard deviation: 309.61

Sample information

Sample name: archaeal virus HSTV-2
Virus: HSTV-2

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Number of particles: 4930

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 62000.0
Defocus max (nm): 2,900.00
Defocus min (nm): 700.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: 20mM Tris-HCl
Specimen support: Quantifoil holey carbon film on 300 mesh copper grid.
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE