EMD-2234 Experiments and Validation

Electron cryo-microscopy of a head-tailed virus infecting extremely halophilic archaea

Single particle reconstruction
Overview of EMD-2234
Sample name: archaeal virus HVTV-1
Organism: HVTV-1
Related EM entry by publication: EMD-2235

Map parameters

Minimum density: -1,731.00
Maximum density: 1,166.00
Average density: -770.235
Standard deviation: 234.181
Recommended contour level: -500.00 (emdb)

Sample information

Sample name: archaeal virus HVTV-1
Virus: HVTV-1

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Number of particles: 3530

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 62000.0
Defocus max (nm): 2,900.00
Defocus min (nm): 400.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: 20mM Tris-HCl
Specimen support: Quantifoil holey carbon film on 300 mesh copper grid.
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE