Single particle reconstruction
8.5Å resolution

Subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

Map released:
Last modified:
Overview of EMD-2233
Source organism: Escherichia coli [562]
Fitted atomic models: 3zbi, 3zbj
Related in-frame EM entry: EMD-2232
Related EM entry by publication: EMD-2232
Primary publication:
Structure of a bacterial type IV secretion core complex at subnanometre resolution.
Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV
EMBO J. 32 1195-1204 (2013)
PMID: 23511972

Function and Biology Details

Sample name: traN/traO/traF complex encoded by pKM101 digested with 0.02 mg ml-1 of elastase for 40 min at room temperature
Proteins: traF, traO, traN

Experimental Information Details

Resolution: 8.5Å
Resolution method: FSC 0.5
Applied symmetry: C14
Reconstruction software: imagic
Microscope: FEI TECNAI 12