EMD-2226 Experiments and Validation

Single particle analysis of recombinant human anaphase promoting complex (APC/C)

Single particle reconstruction
Overview of EMD-2226
Sample name: recombinant human APC/C
Organism: Homo sapiens

Map parameters

Recommended contour level: 3.25 (author)
Number of grid points: 140 × 140 × 140
Voxel size: 3.47 × 3.47 × 3.47 Å
Minimum density: -17.873
Maximum density: 19.055
Average density: 0.054
Standard deviation: 0.884

Sample information

Sample name: recombinant human APC/C
Proteins: Apc1, Apc5, Apc15, Apc10, Apc8, Apc13, Apc2, Apc4, Apc11, Apc3, Apc16, Cdc26, Apc7, Apc6

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Spider, Imagic
Number of particles: 5639

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 100.0
Nominal magnification: 50000.0
Defocus min (nm): 1,200.00
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Details: on recording each adjacent boxes of 2x2 pixels were averaged

Specimen

Preparation
Specimen state: particle
Buffer: 20 mM HEPES NaOH pH 8.0, 200 mM NaCl, 2 mM DTT
Staining procedure: grids stained with 2% (w/v) uranyl acetate
Specimen support: Quantifoil R1.2/1.3 grids coated with a thin layer of carbon
Vitrification
Apparatus: NONE
Cryogen: NONE