EMD-2195
Characterization of the insertase for beta-barrel proteins of the outer mitochondrial membrane. 3-D reconstruction of the TOB complex
EMD-2195
Single-particle14.7 Å
Deposition: 11/09/2012
Map released: 14/11/2012
Last modified: 17/07/2013
Name: TOB complex mitochondrial outer membrane complex required for inserting beta-barrel proteins into the outer membrane.
Summary:
TOB complex mitochondrial outer membrane complex required for inserting beta-barrel proteins into the outer membrane.
Number of unique components: 3
Oligomeric state: heterotrimer with one subunit each Tob37,Tob38 and Tob55
Details: The complexes were monodisperse.
Name: Number of unique components: 3
Oligomeric state: heterotrimer with one subunit each Tob37,Tob38 and Tob55
Details: The complexes were monodisperse.
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
140 kDa | 140 kDa | Blue native gel electrophoresis and Isotope dilution mass spectroscopy analysis of bands isolated from BNGE gels. |
Tob37
Number of copies: 1
Oligomeric state: monmer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Name: Number of copies: 1
Oligomeric state: monmer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Natural source
Organism: Neurospora crassa
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
48 kDa | 48 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
- | - | - | - |
Tob38
Number of copies: 1
Oligomeric state: monomer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Name: Number of copies: 1
Oligomeric state: monomer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Natural source
Organism: Neurospora crassa
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
37 kDa | 37 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
- | - | - | - |
Tob55
Number of copies: 1
Oligomeric state: monomer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Name: Number of copies: 1
Oligomeric state: monomer
Details: The TOB complex contains one copy each of T0b37, Tob38 and Tob55
Natural source
Organism: Neurospora crassa
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Strain: Tob37HT
Organelle: mitochondria
Cellular location: outer membrane
Molecular weight
Experimental | Theoretical | Method |
---|---|---|
54 kDa | 54 kDa | - |
Recombinant Expression
Organism | Strain | Cell | Plasmid |
---|---|---|---|
- | - | - | - |