Single particle reconstruction
25Å resolution

human RNA polymerase II in complex with scAlu RNA

Map released:
Last modified:
Overview of EMD-2188
Source organism: Homo sapiens [9606]
Related EM entries by publication: EMD-2190, EMD-2191, EMD-2192, EMD-2193, EMD-2194, EMD-2189
Primary publication:
Structural insights into transcriptional repression by noncoding RNAs that bind to human Pol II.
Kassube SA, Fang J, Grob P, Yakovchuk P, Goodrich JA, Nogales E
J.MOL.BIOL. 425 3639-3648 (2013)
PMID: 22954660

Function and Biology Details

Sample name: human RNA polymerase II in complex with scAlu RNA
Nucleic acid: scAlu
Proteins: Rpb1, Rpb12, Rpb11, Rpb10, Rpb9, Rpb8, Rpb7, Rpb6, Rpb5, Rpb2, Rpb4, Rpb3

Experimental Information Details

Resolution: 25Å
Resolution method: FSC 0.5
Applied symmetry: C1
Reconstruction software: EMAN2, FREALIGN
Microscope: FEI TECNAI 20
Detector: KODAK SO-163 FILM