EMD-2159 Experiments and Validation

Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37

Single particle reconstruction
Overview of EMD-2159
Sample name: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37
Organism: Yersinia phage phiR1-37
Related EM entry by publication: EMD-2160

Map parameters

Recommended contour level: 12,000.00 (author)
Number of grid points: 401 × 401 × 401
Voxel size: 4.52 × 4.52 × 4.52 Å
Minimum density: -17,749.00
Maximum density: 32,443.00
Average density: 1,168.085
Standard deviation: 5,414.708

Sample information

Sample name: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37
Virus: Yersinia phage phiR1-37

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Number of particles: 1028
CTF correction: Each micrograph

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 15.0
Nominal CS (mm): 2.00
Nominal magnification: 62000.0
Defocus max (nm): 5,760.00
Defocus min (nm): 500.00
Holder model: GATAN LIQUID NITROGEN
Details: Low dose conditions
Acquisition
Number of images: 297
Scanner model: ZEISS SCAI

Specimen

Preparation
Specimen state: particle
Buffer: 50 mM Tris pH 7.8, 10 mM MgSO4
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE