EMD-2151 Experiments and Validation

Electron cryo-microscopy of escrt-III helical polymer

Helical reconstruction
Overview of EMD-2151
Sample name: ESCRT-III Chmp2a - Chmp3 co-polymer
Organism: Homo sapiens

Map parameters

Recommended contour level: 1.40 (author)
Number of grid points: 200 × 200 × 200
Voxel size: 3.59 × 3.59 × 3.59 Å
Minimum density: -3.938
Maximum density: 5.16
Average density: 0.024
Standard deviation: 0.929

Sample information

Sample name: ESCRT-III Chmp2a - Chmp3 co-polymer
Proteins: chmp2a, chmp3

Validation information

Experimental information

 

Image processing

Software: ctffind, bsof, spider, imagic
Reconstruction protocol: ihrsr
CTF correction: each particle

Imaging

Session
Microscope model: FEI TECNAI F30
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 15.0
Nominal CS (mm): 2.30
Nominal magnification: 39000.0
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Number of images: 78
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: filament
Buffer: 20 mM Hepes pH 7.6, 150 mM NaCl
Specimen support: 400 mesh copper quantifoil grid
Aggregation parameters (helical parameters)
Handedness: RIGHT HANDED
Phi increment (degrees): 11.9
Azimuthal (Z) increment (Å): 6.0300000000000002
Rotational symmetry: C6
Vitrification
Apparatus: FEI VITROBOT MARK II
Cryogen: ETHANE
Humidity: 100