EMD-2111 Experiments and Validation

Negative structure of closed conformation of crm1

Single particle reconstruction
Overview of EMD-2111
Sample name: crm1 closed conformation
Organism: Chaetomium thermophilum
Related EM entry by publication: EMD-2110

Map parameters

Minimum density: -0.21
Maximum density: 1.1
Average density: 0
Standard deviation: 0.04
Recommended contour level: 0.04 (emdb)

Sample information

Sample name: crm1 closed conformation
Protein: crm1

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: imagic
Number of particles: 13000
Reconstruction protocol: Cross-common-lines
CTF correction: each particle

Imaging

Session
Microscope model: FEI/PHILIPS CM200FEG
Electron source: FIELD EMISSION GUN
Nominal magnification: 155000.0
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Number of images: 300

Specimen

Preparation
Specimen state: particle
Buffer: 150 mM NaCl, 20 mM HEPES/NaOH pH 7.5, 2 mM MgCl2, 4 mM DTT
Staining procedure: Grids with adsorbed protein floated on 2% w/v uranyl formate for 60 seconds
Specimen support: 200 mesh copper grid with thin carbon support, freshly floated on protein solution
Vitrification
Apparatus: NONE
Cryogen: NONE