Single particle reconstruction
4.5Å resolution

Cryo-EM structure of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon with bound substrate

Map released:
Last modified:
Overview of EMD-2097
Source organism: Methanothermobacter marburgensis [145263]
Fitted atomic model: 3zfs
Related in-frame EM entry: EMD-2096
Related EM entry by publication: EMD-2096
Primary publication:
De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy.
Mills DJ, Vitt S, Strauss M, Shima S, Vonck J
ELIFE 2 e00218-e00218 (2013)
PMID: 23483797

Function and Biology Details

Sample name: F420-reducing [NiFe] hydrogenase Frh with bound substrate
Protein: F420-reducing [NiFe] hydrogenase

Experimental Information Details

Resolution: 4.5Å
Resolution method: feature-based
Applied symmetry: T
Reconstruction software: EMAN2
Microscope: FEI POLARA 300
Detector: KODAK SO-163 FILM