EMD-2076
CryoEM icosahedral reconstruction of AHSV4
EMD-2076
Single-particle14.4 Å
Deposition: 18/04/2012
Map released: 26/09/2012
Last modified: 20/02/2013
Concentration: 0.2
mg/mL
Buffer
pH: 8.5
Details: 2mM Tris-HCl, pH 8.5
Details: 2mM Tris-HCl, pH 8.5
Grid
Details: Quantifoil 200 mesh holey carbon
Vitrification
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.8 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.8 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 7 µm
Number of real images: 307
Average electron dose per image: 18 e/Å2
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 7 µm
Number of real images: 307
Average electron dose per image: 18 e/Å2
Final
reconstruction
Resolution: 14.4
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1633
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1633
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
I
Software
[1]
Name | Version | Details |
---|---|---|
AUTO3DEM | - | - |
CTF correction
Details:Each micrograph
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: AHSV4 filled
Details: ::::EMDATABANK.org::::EMD-2076::::
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: AHSV4 filled
Details: ::::EMDATABANK.org::::EMD-2076::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 401 | 401 | 401 |
Origin | -200 | -200 | -200 |
Spacing | 401 | 401 | 401 |
Voxel size | 2.8 Å | 2.8 Å | 2.8 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 3000.0 | EMDB |