EMD-2076

Single-particle
14.4 Å
EMD-2076 Deposition: 18/04/2012
Map released: 26/09/2012
Last modified: 20/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2076

CryoEM icosahedral reconstruction of AHSV4

EMD-2076

Single-particle
14.4 Å
EMD-2076 Deposition: 18/04/2012
Map released: 26/09/2012
Last modified: 20/02/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.2 mg/mL
Buffer
pH: 8.5
Details: 2mM Tris-HCl, pH 8.5
Grid
Details: Quantifoil 200 mesh holey carbon
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2 mm
Nominal defocus: 0.8 µm - 5.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 7 µm
Number of real images: 307
Average electron dose per image: 18 e/Å2
Image processing [1]
Final reconstruction
Resolution: 14.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 1633
Algorithm: OTHER
Applied Symmetry
Point group: I
Software [1]
Name Version Details
AUTO3DEM - -
CTF correction
Details:Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: AHSV4 filled
Details: ::::EMDATABANK.org::::EMD-2076::::
Geometry
X Y Z
Dimensions 401 401 401
Origin -200 -200 -200
Spacing 401 401 401
Voxel size 2.8 Å 2.8 Å 2.8 Å
Contour list
Primary Level Source
True 3000.0 EMDB