EMD-2074 Experiments and Validation

map of the complex of the HA7 Fab with avian flu virus hemagglutinin by negative staining EM

Single particle reconstruction
Overview of EMD-2074
Sample name: the complex of Fab Fragment of HA7 monoclonal antibody and avian influenza virus hemagglutinin
Organisms: Mus musculus, unidentified influenza virus

Map parameters

Minimum density: -10.621
Maximum density: 13.964
Average density: 0.00
Standard deviation: 1.00
Recommended contour level: 1.80 (author)

Sample information

Sample name: the complex of Fab Fragment of HA7 monoclonal antibody and avian influenza virus hemagglutinin
Proteins: Fab Fragment of HA7 monoclonal antibody, Avian influenza virus hemagglutinin

Validation information

Experimental information

 

Image processing

Applied symmetry: C3
Software: EMAN2
Number of particles: 3172
Reconstruction protocol: Cross-common lines
CTF correction: Each particle
Details: C3 symmetry was imposed in refinement Image processing was done using EMAN2.The particles were selected by a semi-automatic selection using e2boxer.

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Nominal CS (mm): 2.00
Defocus max (nm): 3,600.00
Defocus min (nm): 1,200.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Number of images: 53

Specimen

Preparation
Specimen state: particle
Buffer: 50mM NaCl, 10mM Tris-HCL,1mM CaCl2, pH7.4
Staining procedure: Grids with adsorbed protein floated on 2% w/v uranyl formate for 30 seconds
Specimen support: 300 mesh copper grid with carbon support, glow discharged in vacuumed air
Vitrification
Apparatus: NONE
Cryogen: NONE