EMD-2025 Experiments and Validation

CryoEM 3D reconstruction of full Triatoma Virus

Single particle reconstruction
Overview of EMD-2025
Sample name: Full Triatoma Virus
Organism: Triatoma virus
Related EM entry by publication: EMD-5380, EMD-5379

Map parameters

Minimum density: -122
Maximum density: 212
Average density: 5.68
Standard deviation: 30.8
Recommended contour level: 37 (author)

Sample information

Sample name: Full Triatoma Virus
Virus: Triatoma virus

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: EM3DR2
Number of particles: 1716

Imaging

Session
Microscope model: JEOL 2010F
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 1.4
Nominal magnification: 40000.0
Defocus max (nm): 4,000
Defocus min (nm): 1,200
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 25
Scanner model: ZEISS SCAI
Sampling interval (μm): 7.0

Specimen

Preparation
Specimen state: particle
Buffer: NMT (10 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl)
Specimen support: 400 mesh quantifoil grid
Vitrification
Apparatus: FEI VITROBOT
Cryogen: ETHANE
Details: Vitrification instrument: Vitrobot FEI