EMD-1933

Single-particle
10.3 Å
EMD-1933
3D View Gallery
Deposition: 18/07/2011
Map released: 25/07/2012
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1933
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3zue summary report (pdf.gz) Map and 3zue FULL report (pdf.gz)
EMICSS annotations (xml)

wt Rabbit Hemorrhagic Disease Virus (RHDV)capsid

EMD-1933

Single-particle
10.3 Å
EMD-1933
3D View Gallery
Deposition: 18/07/2011
Map released: 25/07/2012
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 6.0
Details: 200 mM Na2PO4, 100 mM NaCl
Staining
Type: NEGATIVE
Details: Samples were applied to grids, blotted and plunged into liquid ethane
Grid
Details: R 2/2 Quantifoil grids
Vitrification
Cryogen name: ETHANE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.26 mm
Nominal defocus: 0.8 µm - 3.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 116
Average electron dose per image: 10 e/Å2
Bits per pixel: 8.0
Image processing [1]
Final reconstruction
Resolution: 10.3 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 5011
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Xmipp - -
CTF correction
Details:Phase flipping & amplitude decay
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D structure of wt VP1 RHDV capsid
Details: ::::EMDATABANK.org::::EMD-1933::::
⬡ Geometry
X Y Z
Dimensions 219 219 219
Origin -109 -109 -109
Spacing 219 219 219
Voxel size 2.8 Å 2.8 Å 2.8 Å
Contour list
Primary Level Source
True 3.47 AUTHOR