EMD-1906
Tomographic reconstruction of unstained, frozen-hydrated thylakoid membranes from spinach chloroplasts.
EMD-1906
Tomography45.0 Å
Deposition: 08/06/2011
Map released: 14/06/2012
Last modified: 02/10/2013
Method: Tomography
Concentration: 2
mg/mL
Buffer
pH: 6.5
Details: 20mM MES, 5mM MgCl2, 15mM NaCl, pH6.5, 150mM sorbitol.
Details: 20mM MES, 5mM MgCl2, 15mM NaCl, pH6.5, 150mM sorbitol.
Staining
Type:
NEGATIVE
Details: No stain.
Details: No stain.
Grid
Details: Quantifoil 400mesh carbon grids with 1.2 micron holes.
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 8.0 µm - 8.0 µm
Nominal magnification: 34000.0
Calibrated magnification: 33998.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Single tilt
Alignment procedure: LEGACY (Astigmatism: Astigmatism corrected by live FFT., Electron beam tilt params: )
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 8.0 µm - 8.0 µm
Nominal magnification: 34000.0
Calibrated magnification: 33998.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Single tilt
Alignment procedure: LEGACY (Astigmatism: Astigmatism corrected by live FFT., Electron beam tilt params: )
Temperature
Minimum: 96
K
Average: 97 K
Maximum: 98 K
Average: 97 K
Maximum: 98 K
Image Recording
[1]
Sampling interval:
15
µm
Number of real images: 57
Average electron dose per image: 45 e/Å2
Number of real images: 57
Average electron dose per image: 45 e/Å2
Tilt Series
[1]
Axis 1 | Axis 2 | |||||
---|---|---|---|---|---|---|
Min. | Max. | Inc. | Min. | Max. | Inc. | Rotation |
-65 ° | 49 ° | 1 ° | - | - | - | - |
Details: 1 degree increments above 45 deg, 2.5 degree increments below this.
Final
reconstruction
CTF correction
Details:None
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Tomographic reconstruction of photosynthetic membranes from spinach chloroplasts resuspended in an isotonic buffer and imaged in an unstained frozen-hydrated state.
Details: ::::EMDATABANK.org::::EMD-1906::::
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Tomographic reconstruction of photosynthetic membranes from spinach chloroplasts resuspended in an isotonic buffer and imaged in an unstained frozen-hydrated state.
Details: ::::EMDATABANK.org::::EMD-1906::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 991 | 991 | 161 |
Origin | -1 | -1 | 4 |
Spacing | 991 | 991 | 161 |
Voxel size | 17.6 Å | 17.6 Å | 17.6 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 150.0 | AUTHOR |