EMD-1904 Experiments and Validation

Labeling and Localization of the Herpes Simplex Virus Capsid Protein UL25 and Its Interaction with the Two Triplexes Closest to the Penton.

Single particle reconstruction
Overview of EMD-1904
Sample name: HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50.
Organism: Human herpesvirus 1 strain KOS

Map parameters

Recommended contour level: 0.10 (author)
Number of grid points: 642 × 642 × 642
Voxel size: 2.12 × 2.12 × 2.12 Å
Minimum density: -3.733
Maximum density: 4.352
Average density: -0.00
Standard deviation: 1.00

Sample information

Sample name: HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50.
Virus: Human herpesvirus 1 strain KOS

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Reconstruction protocol: Polar Fourier Transform
CTF correction: Phase flip each particle

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 29000.0
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 6.3499999999999996

Specimen

Preparation
Specimen state: particle
Buffer: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: OTHER
Details: Vitrification instrument: Vitrobot mark III. Cryogen was an equal mix of ethane-propane.