EMD-1902 Experiments and Validation

The HSV-1 UL17 protein is the second constituent of the capsid vertex specific component (CVSC) required for DNA packaging and retention.

Single particle reconstruction
Overview of EMD-1902
Sample name: HSV-1 wild type C-capsid (KOS).
Organism: Human herpesvirus 1 strain KOS
Related EM entry by publication: EMD-1903

Map parameters

Recommended contour level: 0.60 (author)
Number of grid points: 642 × 642 × 642
Voxel size: 2.12 × 2.12 × 2.12 Å
Minimum density: -6.368
Maximum density: 6.073
Average density: 0.00
Standard deviation: 1.00

Sample information

Sample name: HSV-1 wild type C-capsid (KOS).
Virus: Human herpesvirus 1 strain KOS

Validation information

Experimental information

 

Image processing

Applied symmetry: I
Software: AUTO3DEM
Number of particles: 3973
Reconstruction protocol: Polar Fourier Transform
CTF correction: Phase flip each particle

Imaging

Session
Microscope model: FEI TECNAI F20
Electron source: FIELD EMISSION GUN
Nominal CS (mm): 2.00
Nominal magnification: 29000.0
Defocus max (nm): 2,730.00
Defocus min (nm): 1,630.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Number of images: 48
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 6.3499999999999996

Specimen

Preparation
Specimen state: particle
Buffer: 500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification
Apparatus: FEI VITROBOT MARK III
Cryogen: ETHANE
Humidity: 85
Protocol: 7.5 second blot before plunging
Details: Vitrification instrument: Vitrobot mark III