EMD-1856 Experiments and Validation

Automatic Recovery of Missing Amplitudes and Phases in Tilt-Limited Electron Crystallography of 2D Crystals

Overview of EMD-1856
Sample name: Bacteriorhodopsin
Organism: Halobacterium salinarum

Map parameters

Minimum density: -0.042
Maximum density: 0.058
Average density: 0.00
Standard deviation: 0.008
Recommended contour level: 0.012 (author)

Sample information

Sample name: Bacteriorhodopsin
Protein: Bacteriorhodopsin

Validation information

Experimental information

 

Image processing

Reconstruction protocol: PCO
CTF correction: Phase flipping and Electron Diffraction
Details: 3D-PCO reconstructed maps, generated from phase-flipped raw 2D kimura, 97 crystallographic dataset.

Imaging

Session
Microscope model: JEOL KYOTO-3000SFF
Electron source: OTHER
Holder model: JEOL
Details: The full details of the original dataset can be found from PDB entry 1at9

Specimen

Preparation
Specimen state: twoDArray
Aggregation parameters (crystal parameters)
Plane group: P 3
Vitrification
Apparatus: NONE
Cryogen: NITROGEN