EMD-1615 Experiments and Validation

Three-dimensional structure of YidC bound to the translating ribosome

Single particle reconstruction
Overview of EMD-1615
Sample name: YidC bound to a translating ribosome
Organisms: Escherichia coli, Escherichia coli K-12
Related EM entry by publication: EMD-1616

Map parameters

Recommended contour level: 40.00 (author)
Number of grid points: 108 × 108 × 108
Voxel size: 3.76 × 3.76 × 3.76 Å
Minimum density: -191.38
Maximum density: 342.407
Average density: 2.55
Standard deviation: 27.298

Sample information

Sample name: YidC bound to a translating ribosome
Protein: YidC
Ribosome: Ribosome nascent chain complex

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: Imagic-5 Spider
Number of particles: 24395
Reconstruction protocol: Projection matching
CTF correction: Each image

Imaging

Session
Microscope model: FEI TECNAI 20
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 15.0
Nominal magnification: 50000.0
Defocus max (nm): 4,000.00
Defocus min (nm): 1,000.00
Tilt max (degrees): 0.00
Tilt min (degrees): 0.00
Holder model: GATAN LIQUID NITROGEN
Acquisition
Scanner model: NIKON SUPER COOLSCAN 9000
Sampling interval (μm): 12.699999999999999

Specimen

Preparation
Specimen state: particle
Specimen support: 200 mesh copper
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE
Details: Vitrification instrument: Plunger