EMDB EMD-1055

Single particle reconstruction
9Å resolution

Locking and unlocking of ribosomal motions.

Map released:
Last modified:
Overview of EMD-1055
Source organism: Escherichia coli [562]
Fitted atomic models: 1qza, 1qzd, 1r2x
Related EM entry by publication: EMD-1056, EMD-1395
Primary publication:
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy.
Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J
NAT.STRUCT.MOL.BIOL. 10 899-906 (2003)
PMID: 14566331

Function and Biology Details

Sample name: 70S ribosome from E. coli complex 70S-fMet-tRNA-Phe-tRNA-EF-Tu-GDP-kirromycin.
Nucleic acids: fMet-tRNAfMet, Phe-tRNAPhe, deacylated tRNA
Protein: Elongation Factor Tu
Ribosome: 70S from E. coli

Experimental Information Details

Resolution: 9Å
Resolution method: FSC cut-off at 0.15
Applied symmetry: C1
Reconstruction software: Spider
Microscope: FEI TECNAI F20
Detector: KODAK SO-163 FILM