EMD-1048

Single-particle
12.0 Å
EMD-1048
3D View Gallery
Deposition: 19/05/2003
Map released: 19/09/2003
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1048
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 1pdf summary report (pdf.gz) Map and PDB model 1pdi summary report (pdf.gz) Map and PDB model 1pdj summary report (pdf.gz) Map and PDB model 1pdl summary report (pdf.gz) Map and PDB model 1pdm summary report (pdf.gz) Map and PDB model 1pdp summary report (pdf.gz) Map and PDB model 2fl8 summary report (pdf.gz) Map and PDB model 3h3w summary report (pdf.gz) Map and 1pdf FULL report (pdf.gz) Map and 1pdi FULL report (pdf.gz) Map and 1pdj FULL report (pdf.gz) Map and 1pdl FULL report (pdf.gz) Map and 1pdm FULL report (pdf.gz) Map and 1pdp FULL report (pdf.gz) Map and 2fl8 FULL report (pdf.gz) Map and 3h3w FULL report (pdf.gz)
EMICSS annotations (xml)

Three-dimensional structure of bacteriophage T4 baseplate.

EMD-1048

Single-particle
12.0 Å
EMD-1048
3D View Gallery
Deposition: 19/05/2003
Map released: 19/09/2003
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5 mg/mL
Buffer
pH: 7.0
Details: Water
Grid
Details: Holey carbon
Vitrification
Cryogen name: ETHANE
Method: Blot for 2 seconds before plunging
Microscopy [1]
Microscope: FEI/PHILIPS CM300FEG/T
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 1.2 µm - 5.0 µm
Nominal magnification: 45000.0
Calibrated magnification: 47000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: 626 Single Tilt Cryotransfer System
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 14 µm
Number of real images: 15
Average electron dose per image: 25 e/Å2
Bits per pixel: 8.0
Details: Images were scanned at 7 micron per pixel and averaged 2x2 to give 14 micron per pixel
Image processing [1]
Final reconstruction
Resolution: 12.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 945
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C6
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Map contains the baseplate and the proximal part of the tail tube
Details: ::::EMDATABANK.org::::EMD-1048::::
⬡ Geometry
X Y Z
Dimensions 196 196 196
Origin -98 -98 -97
Spacing 196 196 196
Voxel size 2.97872 Å 2.97872 Å 2.97872 Å
Contour list
Primary Level Source
True 1.45 AUTHOR