EMD-0290
Immature M-PMV capsid hexamer structure in intact virus particles
EMD-0290
Subtomogram averaging7.2 Å
Deposition: 12/10/2018
Map released: 05/12/2018
Last modified: 19/12/2018
Details: Purified virus solution was inactivated and diluted 1:1 with PBS containing 10 nm colloidal gold.
Buffer
pH: 6.0
Grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 288 K
Instrument: FEI VITROBOT MARK II
Chamber humidity: 95%
Chamber temperature: 288 K
Instrument: FEI VITROBOT MARK II
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.7 mm
Nominal defocus: 2.0 µm - 4.5 µm
Nominal magnification: 105000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 50.0 µm
Nominal CS: 2.7 mm
Nominal defocus: 2.0 µm - 4.5 µm
Nominal magnification: 105000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: ZEMLIN TABLEAU
Specialist optics
Energy filter
Image Recording
[1]
Detector model:
GATAN K2 QUANTUM (4k x 4k)
Detector mode: SUPER-RESOLUTION
Dimensions: 3710 pixel x 3838 pixel
Sampling interval: 5.0 µm
Frames per image: 1-6
Number of grids: 1
Average exposure time: 0.6 s
Average electron dose per image: 2.0 e/Å2
Details: Dose fluctuation was caused by the ring collapse of FEG during data collection.
Detector mode: SUPER-RESOLUTION
Dimensions: 3710 pixel x 3838 pixel
Sampling interval: 5.0 µm
Frames per image: 1-6
Number of grids: 1
Average exposure time: 0.6 s
Average electron dose per image: 2.0 e/Å2
Details: Dose fluctuation was caused by the ring collapse of FEG during data collection.
Final
reconstruction
Resolution: 7.2
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Resolution method: FSC 0.143 CUT-OFF
⌯ Applied Symmetry
Point group:
C6
⦩ Final angle assignment
Extraction
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 192 | 192 | 192 |
Origin | 0 | 0 | 0 |
Spacing | 192 | 192 | 192 |
Voxel size | 1.35 Å | 1.35 Å | 1.35 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.118 | AUTHOR |