EMDB EMD-0289

Single particle reconstruction
3.3Å resolution

Structure of a functional obligate respiratory supercomplex from Mycobacterium smegmatis

Map released:
Overview of EMD-0289
Source organism: Mycobacterium smegmatis MC2 155 [246196]
Fitted atomic model: 6hwh
To be published

Function and Biology Details

Sample name: Respiratory Supercomplex from Mycobacterium smegmatis
Ligands: FE2/S2 (INORGANIC) CLUSTER, CARDIOLIPIN, MENAQUINONE-9, COPPER (II) ION, HEME-AS, HEME C, PROTOPORPHYRIN IX CONTAINING FE
Proteins: Respiratory Supercomplex from Mycobacterium smegmatis, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Co-purified unknown transmembrane helices built as polyALA, Co-purified unknown transmembrane helices built as polyALA, Co-purified unknown peptide built as polyALA, Co-purified unknown peptide built as polyALA, Cytochrome bc1 complex cytochrome c subunit, Cytochrome c oxidase subunit 2, MSMEG_4693, Uncharacterized protein MSMEG_4692/MSMEI_4575, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide 4, Cytochrome c oxidase subunit 3, Ubiquinol-cytochrome C reductase QcrB

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)