EMD-0226

Single-particle
4.2 Å
EMD-0226
3D View Gallery
Deposition: 30/08/2018
Map released: 07/11/2018
Last modified: 14/09/2022
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0226
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6hio summary report (pdf.gz) Map and 6hio FULL report (pdf.gz)
EMICSS annotations (xml)

Mouse serotonin 5-HT3 receptor, serotonin-bound, I1 conformation

EMD-0226

Single-particle
4.2 Å
EMD-0226
3D View Gallery
Deposition: 30/08/2018
Map released: 07/11/2018
Last modified: 14/09/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 60.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 16660
⌯ Applied Symmetry
Point group: C5
Startup model [1]
Type: PDB ENTRY
PDB model: 4PIR
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Mouse serotonin 5-HT3 receptor, serotonin-bound, I1 conformation
⬡ Geometry
X Y Z
Dimensions 240 240 240
Origin 0 0 0
Spacing 240 240 240
Voxel size 1.039 Å 1.039 Å 1.039 Å
Contour list
Primary Level Source
True 0.0351 AUTHOR