EMD-0196 Function and Biology

Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Single particle reconstruction
3.58Å resolution
Overview of EMD-0196
Sample name: nanodisc-reconsituted ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab
Number of unique components: 8

nanodisc-reconsituted ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Protein type
Name: nanodisc-reconsituted ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

ABCG2 E211Q mutant [Homo sapiens]

Protein type
Name: ABCG2 E211Q mutant
Expression system
Expression system: Homo sapiens
Cell line: HEK293 EBNA

5D3-Fab [Mus musculus]

Protein type
Name: 5D3-Fab
Expression system
Expression system: Mus musculus

ATP-binding cassette sub-family G member 2 [Homo sapiens]

Protein type
Name: ATP-binding cassette sub-family G member 2
Theoretical weight (MDa): 0.073
Number of copies: 2
Expression system
Expression system: Homo sapiens

5D3-Fab light chain [Mus musculus]

Protein type
Name: 5D3-Fab light chain
Theoretical weight (MDa): 0.024
Number of copies: 2
Expression system
Expression system: Mus musculus

5D3-Fab heavy chain [Mus musculus]

Protein type
Name: 5D3-Fab heavy chain
Theoretical weight (MDa): 0.024
Number of copies: 2
Expression system
Expression system: Mus musculus

N-ACETYL-D-GLUCOSAMINE

Ligand type
Name: N-ACETYL-D-GLUCOSAMINE
Theoretical weight (MDa): 0
Number of copies: 4

estrone 3-sulfate

Ligand type
Name: estrone 3-sulfate
Theoretical weight (MDa): 0
Number of copies: 1