EMDB EMD-0196

Single particle reconstruction
3.58Å resolution

Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Map released:
Last modified:
Overview of EMD-0196
Source organisms:
Fitted atomic model: 6hco
Related EM entry by publication: EMD-0190
Primary publication:
Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states.
Manolaridis I, Jackson SM, Taylor NMI, Kowal J, Stahlberg H, Locher KP
Nature 563 426-430 (2018)
PMID: 30405239

Function and Biology Details

Sample name: nanodisc-reconsituted ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab
Ligands: N-ACETYL-D-GLUCOSAMINE, estrone 3-sulfate
Proteins: nanodisc-reconsituted ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab, ABCG2 E211Q mutant, 5D3-Fab, ATP-binding cassette sub-family G member 2, 5D3-Fab light chain, 5D3-Fab heavy chain

Experimental Information Details

Resolution: 3.58Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)