EMDB EMD-0190

Single particle reconstruction
3.09Å resolution

Cryo-EM structure of the ABCG2 E211Q mutant bound to ATP and Magnesium

Map released:
Overview of EMD-0190
Source organism: Homo sapiens [9606]
Fitted atomic model: 6hbu
Related EM entry by publication: EMD-0196
Primary publication:
Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states
Manolaridis I, Jackson SM, Taylor NMI, Kowal J, Stahlberg H, Locher KP
Nature - (2018)

Function and Biology Details

Sample name: inhibitor-bound ABCG2
Ligands: ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Proteins: inhibitor-bound ABCG2, A mutant (E211Q) of the ATP-binding cassette sub-family G member 2, ATP-binding cassette sub-family G member 2

Experimental Information Details

Resolution: 3.09Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)