EMDB EMD-0100

Single particle reconstruction
3.5Å resolution

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-3 (TI-POST-3)

Map released:
Overview of EMD-0100
Source organisms:
Fitted atomic model: 6gz5
To be published

Function and Biology Details

Sample name: translocation intermediate TI-POST-3
Ligands: MAGNESIUM ION, ZINC ION, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
Nucleic acids: 28S ribosomal RNA, P/P-site-tRNA, mRNA, E/E-site-tRNA, 18S ribosomal RNA, 5.8S ribosomal RNA, 5S ribosomal RNA
Proteins: translocation intermediate TI-POST-3, translocation intermediate TI-POST-3, translocation intermediate TI-POST-3, translocation intermediate TI-POST-3, ribosomal protein uS3, ribosomal protein uS7, ribosomal protein eS10, ribosomal protein eS12, ribosomal protein uS19, ribosomal protein uS9, ribosomal protein eS17, ribosomal protein uS13, ribosomal protein eS19, ribosomal protein uS10, ribosomal protein eS25, ribosomal protein eS28, ribosomal protein uS14, ribosomal protein eS31, ribosomal protein RACK 1, ribosomal protein uS2, ribosomal protein eS1, ribosomal protein uS5, ribosomal protein eS4, ribosomal protein eS6, ribosomal protein eS7, ribosomal protein eS8, ribosomal protein uS4, ribosomal protein uS17, ribosomal protein uS15, ribosomal protein uS11, ribosomal protein eS21, ribosomal protein uS8, ribosomal protein uS12, ribosomal protein eS24, ribosomal protein eS26, ribosomal protein eS27, ribosomal protein eS30, ribosomal protein uL2, ribosomal protein uL3, ribosomal protein uL4, ribosomal protein uL18, ribosomal protein eL6, ribosomal protein uL30, ribosomal protein eL8, ribosomal protein uL6, ribosomal protein uL16, ribosomal protein uL5, ribosomal protein eL13, ribosomal protein eL14, ribosomal protein eL15, ribosomal protein uL13, ribosomal protein uL22, ribosomal protein eL18, ribosomal protein eL19, ribosomal protein eL20, ribosomal protein eL21, ribosomal protein eL22, ribosomal protein uL14, ribosomal protein eL24, ribosomal protein uL23, ribosomal protein uL24, ribosomal protein eL27, ribosomal protein uL15, ribosomal protein eL29, ribosomal protein eL30, ribosomal protein eL31, ribosomal protein eL32, ribosomal protein eL33, ribosomal protein eL34, ribosomal protein uL29, ribosomal protein eL36, ribosomal protein eL37, ribosomal protein eL38, ribosomal protein eL39, ribosomal protein eL40, ribosomal protein eL41, ribosomal protein eL42, ribosomal protein eL43, ribosomal protein eL28, ribosomal protein uL1, ribosomal protein uL11, ribosomal protein uL10, eukaryotic elongation factor 2 (eEF2)

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: SPIDER
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)