EMD-0098

Single-particle
3.6 Å
EMD-0098
3D View Gallery
Deposition: 03/07/2018
Map released: 05/12/2018
Last modified: 30/03/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0098
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6gz3 summary report (pdf.gz) Map and 6gz3 FULL report (pdf.gz)
EMICSS annotations (xml)

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1)

EMD-0098

Single-particle
3.6 Å
EMD-0098
3D View Gallery
Deposition: 03/07/2018
Map released: 05/12/2018
Last modified: 30/03/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average exposure time: 5.0 s
Average electron dose per image: 30.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 32386
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: OTHER
Details:Filtered empty 80S
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Particle selection [1]
Selected Ref. model Method Software Details
270000 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 405 405 405
Origin 0 0 0
Spacing 405 405 405
Voxel size 0.975 Å 0.975 Å 0.975 Å
Contour list
Primary Level Source
True 2.5 AUTHOR