EMDB EMD-0098

Single particle reconstruction
3.6Å resolution

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1)

Map released:
Overview of EMD-0098
Source organisms:
Fitted atomic model: 6gz3
To be published

Function and Biology Details

Sample name: translocation intermediate TI-POST-1
Ligands: MAGNESIUM ION, ZINC ION, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
Nucleic acids: 28S ribosomal RNA, ap/P-site tRNA, mRNA, pe/E-site-tRNA, 18S ribosomal RNA, 5.8S ribosomal RNA, 5S ribosomal RNA
Proteins: translocation intermediate TI-POST-1, Ribosome, mRNA, tRNA, ribosomal protein uS3, Ribosomal protein S5, ribosomal protein eS10, 40S ribosomal protein S12, ribosomal protein uS19, ribosomal protein uS9, ribosomal protein eS17, ribosomal protein uS13, ribosomal protein eS19, ribosomal protein uS10, ribosomal protein eS25, Ribosomal protein S28, ribosomal protein uS14, Ribosomal protein S27a, ribosomal protein RACK 1, ribosomal protein uS2, 40S ribosomal protein S3a, ribosomal protein uS5, ribosomal protein eS4, 40S ribosomal protein S6, 40S ribosomal protein S7, ribosomal protein eS8, Ribosomal protein S9 (Predicted), Ribosomal protein S11, ribosomal protein uS15, ribosomal protein uS11, ribosomal protein eS21, Ribosomal protein S15a, Ribosomal protein S23, ribosomal protein eS24, ribosomal protein eS26, ribosomal protein eS27, ribosomal protein eS30, Ribosomal protein L8, ribosomal protein uL3, ribosomal protein uL4, ribosomal protein uL18, ribosomal protein eL6, ribosomal protein uL30, ribosomal protein eL8, ribosomal protein uL6, Ribosomal protein L10 (Predicted), Ribosomal protein L11, ribosomal protein eL13, ribosomal protein eL14, Ribosomal protein L15, ribosomal protein uL13, ribosomal protein uL22, ribosomal protein eL18, ribosomal protein eL19, ribosomal protein eL20, ribosomal protein eL21, ribosomal protein eL22, Ribosomal protein L23, ribosomal protein eL24, ribosomal protein uL23, Ribosomal protein L26, 60S ribosomal protein L27, ribosomal protein uL15, ribosomal protein eL29, ribosomal protein eL30, ribosomal protein eL31, ribosomal protein eL32, ribosomal protein eL33, ribosomal protein eL34, ribosomal protein uL29, ribosomal protein eL36, Ribosomal protein L37, ribosomal protein eL38, ribosomal protein eL39, ribosomal protein eL40, ribosomal protein eL41, ribosomal protein eL42, ribosomal protein eL43, ribosomal protein eL28, Ribosomal protein, Ribosomal protein L12, ribosomal protein uL10, eukaryotic elongation factor 2 (eEF2)

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)