EMDB EMD-0091

Single particle reconstruction
4.8Å resolution

Structure of a yeast closed complex with distorted DNA (core CCdist)

Map released:
Overview of EMD-0091
Source organism: Baker's yeast [559292]
Fitted atomic model: 6gyl
Related EM entry by publication: EMD-0090, EMD-0092
Primary publication:
Promoter Distortion and Opening in the RNA Polymerase II Cleft.
Dienemann C, Schwalb B, Schilbach S, Cramer P
Mol. Cell - (2018)
PMID: 30472190

Function and Biology Details

Sample name: Pre-initiation complex
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: GAT1 promoter DNA, GAT1 promoter DNA
Proteins: Pre-initiation complex, Polymerase, TIFs, nucleic acids, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, DNA-directed RNA polymerases I, II, and III subunit RPABC4, Transcription initiation factor IIB, TATA-box-binding protein, Transcription initiation factor IIF subunit alpha, Transcription initiation factor IIF subunit beta, Transcription initiation factor IIA large subunit,Transcription initiation factor IIA large subunit, Transcription initiation factor IIA subunit 2, Transcription initiation factor IIE subunit alpha,Tfa1,Tfa1, Transcription initiation factor IIE subunit beta

Experimental Information Details

Resolution: 4.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)