EMDB EMD-0090

Single particle reconstruction
5.1Å resolution

Structure of a yeast closed complex (core CC1)

Map released:
Overview of EMD-0090
Source organism: Baker's yeast [559292]
Fitted atomic model: 6gyk
Related EM entry by publication: EMD-0091, EMD-0092
Primary publication:
Promoter Distortion and Opening in the RNA Polymerase II Cleft.
Dienemann C, Schwalb B, Schilbach S, Cramer P
Mol. Cell - (2018)
PMID: 30472190

Function and Biology Details

Sample name: Yeast pre-initiation complex
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: GAT1 Promoter, GAT1 promoter DNA
Proteins: Yeast pre-initiation complex, RNA polymerase, TFs, Nucleic acids, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, DNA-directed RNA polymerases I, II, and III subunit RPABC4, Transcription initiation factor IIB, TATA-box-binding protein, Transcription initiation factor IIF subunit alpha, Transcription initiation factor IIF subunit beta, Transcription initiation factor IIA large subunit,Transcription initiation factor IIA large subunit, Transcription initiation factor IIA subunit 2

Experimental Information Details

Resolution: 5.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)