EMD-0049

Single-particle
4.0 Å
EMD-0049
3D View Gallery
Deposition: 08/06/2018
Map released: 11/07/2018
Last modified: 08/08/2018
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0049
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6gqv summary report (pdf.gz) Map and 6gqv FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM recosntruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP)

EMD-0049

Single-particle
4.0 Å
EMD-0049
3D View Gallery
Deposition: 08/06/2018
Map released: 11/07/2018
Last modified: 08/08/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Buffer components [5]:
Name Formula Concentration ChEBI
- Hepes-KOH 10.0 mM
- Mg(OAc)2 5.0 mM
- KOAc 40.0 mM
- NH4Cl 10.0 mM
- DTT 1.0 mM
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Details: Blot force 4, blot waiting time 30 s.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average exposure time: 1.5 s
Average electron dose per image: 60.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 86500
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: PDB ENTRY
PDB model: 4UG0
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 1.1 Å 1.1 Å 1.1 Å
Contour list
Primary Level Source
True 0.033 EMDB