EMDB EMD-0047

Single particle reconstruction
4.4Å resolution

Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP/sordarin)

Map released:
Last modified:
Overview of EMD-0047
Source organism: Baker's yeast [559292]
Fitted atomic model: 6gq1
Related EM entries by publication: EMD-0048, EMD-0049, EMD-0055
Primary publication:
Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y
J. Mol. Biol. 430 2677-2687 (2018)
PMID: 29886014

Function and Biology Details

Sample name: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of non-hydrolizable GTP (GMPPCP) and anti-fungal drug (sordarin)
Ligands: ZINC ION, [1R-(1.ALPHA.,3A.BETA.,4.BETA.,4A.BETA.,7.BETA.,7A.ALPHA.,8A.BETA.)]8A-[(6-DEOXY-4-O-METHYL-BETA-D-ALTROPYRANOSYLOXY)METHYL]-4-FORMYL-4,4A,5,6,7,7A,8,8A-OCTAHYDRO-7-METHYL-3-(1-METHYLETHYL)-1,4-METHANO-S-INDACENE-3A(1H)-CARBOXYLIC ACID, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
Nucleic acids: Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 5.8S ribosomal RNA, 5S ribosomal RNA, 18S ribosomal RNA, Transfer RNA - Phe, Messenger RNA
Proteins: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of non-hydrolizable GTP (GMPPCP) and anti-fungal drug (sordarin), 80S ribosome, RNA, 60S acidic ribosomal protein P0, 60S ribosomal protein L12-A, 60S ribosomal protein L2-A, 60S ribosomal protein L3, 60S ribosomal protein L4-A, 60S ribosomal protein L5, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, 60S ribosomal protein L9-A, 60S ribosomal protein L10, 60S ribosomal protein L11-B, 60S ribosomal protein L13-A, 60S ribosomal protein L14-A, 60S ribosomal protein L15-A, 60S ribosomal protein L16-A, 60S ribosomal protein L17-A, 60S ribosomal protein L18-A, 60S ribosomal protein L19-A, 60S ribosomal protein L20-A, 60S ribosomal protein L21-A, 60S ribosomal protein L22-A, 60S ribosomal protein L23-A, 60S ribosomal protein L24-A, 60S ribosomal protein L25, 60S ribosomal protein L26-A, 60S ribosomal protein L27-A, 60S ribosomal protein L28, 60S ribosomal protein L29, 60S ribosomal protein L30, 60S ribosomal protein L31-A, 60S ribosomal protein L32, 60S ribosomal protein L33-A, 60S ribosomal protein L34-A, 60S ribosomal protein L35-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A, 60S ribosomal protein L38, 60S ribosomal protein L39, Ubiquitin-60S ribosomal protein L40, 60S ribosomal protein L41-B, 60S ribosomal protein L42-A, 60S ribosomal protein L43-A, 40S ribosomal protein S0-A, 40S ribosomal protein S1-A, 40S ribosomal protein S2, 40S ribosomal protein S3, 40S ribosomal protein S4-A, 40S ribosomal protein S5, 40S ribosomal protein S6-A, 40S ribosomal protein S7-A, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, 40S ribosomal protein S10-A, 40S ribosomal protein S11-A, 40S ribosomal protein S12, 40S ribosomal protein S13, 40S ribosomal protein S14-B, 40S ribosomal protein S15, 40S ribosomal protein S16-A, 40S ribosomal protein S17-B, 40S ribosomal protein S18-A, 40S ribosomal protein S19-A, 40S ribosomal protein S20, 40S ribosomal protein S21-A, 40S ribosomal protein S22-A, 40S ribosomal protein S23-A, 40S ribosomal protein S24-A, 40S ribosomal protein S25-A, 40S ribosomal protein S26-B, 40S ribosomal protein S27-A, 40S ribosomal protein S28-A, 40S ribosomal protein S29-A, 40S ribosomal protein S30-A, Guanine nucleotide-binding protein subunit beta-like protein, Ubiquitin-40S ribosomal protein S31, Elongation factor 2

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)