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Title:Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Authors:Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Sample:Cas9 loaded with crRNA:tracrRNA and bound to target DNA duplex
Method:Single particle reconstruction (21 angstroms resolution)
Red flagLatest update:2014-03-26
Summary
Status: Released
Deposition date: 2014-01-13
Deposition site: RCSB
Processing site: RCSB
Header release date: 2014-02-12
Map release date: 2014-02-19
Primary citation: Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
SCIENCE (2014) 343, pp. 1247997 [PubMed 24505130] [DOI]
Sample: Cas9 loaded with crRNA:tracrRNA and bound to target DNA duplex
Resolution: 21 Å (determined by FSC 0.5, semi-independent)