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Title:A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5A resolution
Authors:Zhang X, Guo H, Jin L, Czornyj E, Hodes A, Hui WH, Nieh AW, Miller JF, Zhou ZH
Sample:Bordetella bacteriophage
Method:Single particle reconstruction (3.5 angstroms resolution)
Red flagLatest update:2014-01-08
Sample
Sample name: Bordetella bacteriophage
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusBordetella phage BPP-1Bordetella phage BPP-1
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL50 mM Tris-HCl, 250 mM NaCl400 mesh holey carbon film
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IV ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm800 nm2300 nm5900057660FIELD EMISSION GUNKODAK SO-163 FILM mmmanual correction

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
FEI TITAN KRIOS AUTOGRID HOLDER°° eV80 K K K0 mrad25 e/Å212-JUL-2009
Processing
Protocol:projection matching
Software:eLite3D, Frealign
CTF correction:Each particle
Number of particles:39549
Number of class averages:1
Imposed symmetry:I
Resolution by author:3.5 Å
Resolution method:FSC at 0.143 cut-off, feature-based method and R-factor in model refinement
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
8956.35 μm/pixel2NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails