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Title:Pseudorabies GS-2168 B-capsid from cryo-electron microscopy
Authors:Homa FL, Huffman JB, Toropova K, Lopez HR, Makhov AM, Conway JF
Sample:Pseudorabies virus GS-2168 UL25 - null mutant grown on PK15 cells
Method:Single particle reconstruction (14.7 angstroms resolution)
Red flagLatest update:2013-09-04
Sample
Sample name: Pseudorabies virus GS-2168 UL25 - null mutant grown on PK15 cells
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusSuid herpesvirus 1Suid herpesvirus 1Mutant does not package DNA.
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mLTNE: 500mM NaCl, 10mM Tris, 1mM EDTAQuantifoil R2/1, 200 mesh copper, glow discharged for 10-15 secs
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE-PROPANE MIXTURE95%82 KFEI VITROBOT MARK IIIBlot for 6 seconds. ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 20200 kVFLOOD BEAMBRIGHT FIELD2 mm nm nm2900030000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Gatan 626GATAN LIQUID NITROGEN°° eV K K95 K mrad e/Å211-NOV-2008
Processing
Protocol:Fourier methods
Software:AUTO3DEM(v3.07)
CTF correction:Each micrograph
Number of particles:5870
Imposed symmetry:I
Resolution by author:14.7 Å
Resolution method:FSC 0.5
Processing details:Particles were picked with x3dpreprocess.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
416.35 μm/pixel8NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails