EMD-5632

Subtomogram averaging
37.0 Å
EMD-5632 Deposition: 05/04/2013
Map released: 14/08/2013
Last modified: 26/03/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5632

Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgG mutant

EMD-5632

Subtomogram averaging
37.0 Å
EMD-5632 Deposition: 05/04/2013
Map released: 14/08/2013
Last modified: 26/03/2014
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Subtomogram averaging
Aggregation State: Cell
Specimen preparation [1]
Buffer
Details: BSK-II liquid medium supplemented with 6% rabbit serum
Grid
Details: Freshly glow-discharged holey carbon grids
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Method: Bacterial culture was deposited onto freshly glow-discharged, holey carbon grids for 1 min. Grids were blotted with filter paper and then rapidly frozen in liquid ethane, using a homemade gravity-driven plunger apparatus.
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2 mm
Nominal defocus: 6.0 µm - 8.0 µm
Nominal magnification: 31000.0
Specimen holder model: OTHER
Temperature
Minimum: 170 K
Image Recording [1]
Number of real images: 8265
Average electron dose per image: 100 e/Å2
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
-65 ° 65 ° - - - - -
Image processing [1]
Details: The subvolumes of the flagellar motors were extracted computationally from the tomograms and were further aligned in 3D.
Final reconstruction
Resolution: 37.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
Applied Symmetry
Point group: C1
Software [1]
Name Version Details
IMOD, Raptor, Protomo - -
Final 3D classification
Number of classes: 4
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Flagellar motor of a FlgG mutant in B. burgdorferi
Details: ::::EMDATABANK.org::::EMD-5632::::
Geometry
X Y Z
Dimensions 256 256 256
Origin -7 -7 -7
Spacing 256 256 256
Voxel size 5.7 Å 5.7 Å 5.7 Å
Contour list
Primary Level Source
True 2.5 AUTHOR