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Title:Yeast 20S proteasome with C-terminal peptide of yeast Rpt2
Authors:Li X, Kim HM, Cheng Y
Sample:Yeast 20S proteasome
Method:Single particle reconstruction (7.7 angstroms resolution)
Red flagLatest update:2013-06-05
Sample
Sample name: Yeast 20S proteasome
Oligomeric state: 28mer
Experimental molecular weight of the sample: 0.7
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1protein20S proteasome0.7falseSaccharomyces cerevisiaeGST tag was added to C-terminus of beta-2 subunit
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mLQuantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%120 KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1200 nm2100 nm100000FIELD EMISSION GUNTVIPS TEMCAM-F816 (8k x 8k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
CT3500GATAN LIQUID NITROGEN eV K K K mrad25 e/Å2Low-Dose procedure01-JAN-2010
Processing
Software:FREALIGN
CTF correction:Each particle
Number of particles:88013
Imposed symmetry:C2
Resolution by author:7.7 Å
Resolution method:FSC 0.143
Processing details:FREALIGN.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1RYP rigid bodyChimeraREAL