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Title:3D reconstruction of yeast 20S proteasome core particle
Authors:Li X, Kim HM, Cheng Y
Sample:Yeast 20S proteasome
Method:Single particle reconstruction (6.9 angstroms resolution)
Red flagLatest update:2013-06-05
Sample
Sample name: Yeast 20S proteasome
Oligomeric state: 28mer
Experimental molecular weight of the sample: 0.7
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinyeast 20S proteasome0.728falseSaccharomyces cerevisiaeGST tag was added to C terminus of beta-2 subunit
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mLQuantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1200 nm2100 nm100000FIELD EMISSION GUNTVIPS TEMCAM-F816 (8k x 8k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
CT3500GATAN LIQUID NITROGEN eV K K K mrad25 e/Å2Low-Dose procedure01-JAN-2010
Processing
Software:FREALIGN
CTF correction:Each particle
Number of particles:321296
Imposed symmetry:C2
Resolution by author:6.9 Å
Resolution method:FSC 0.143
Processing details:FREALIGN.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1RYP rigid bodyChimeraREAL