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Title:Electron cryo-microscopy of Drosophila melanogaster EF2- and Vig2-bound 80S ribosome
Authors:Anger AM, Armache J-P, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Sample:80S D. melanogaster ribosome
Method:Single particle reconstruction (6.0 angstroms resolution)
Red flagLatest update:2014-07-23
Sample
Sample name: 80S D. melanogaster ribosome
Experimental molecular weight of the sample: 4
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosome with E-tRNA, EF-2, and Vig244falseDrosophila melanogaster
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IVBefore plunging, blot for 3 seconds using two layers of filter paper. ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm1000 nm4000 nm9000090000FIELD EMISSION GUNFEI EAGLE (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN°° eV K K K mrad20 e/Å210-MAR-2011
Processing
Protocol:Projection matching
Software:Spider
CTF correction:each subvolume
Number of particles:134500
Imposed symmetry:C1
Resolution by author:6.0 Å
Resolution method:FSC 0.5
Processing details:The particles were selected using an automatic selection program.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails