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Title:Negatively Stained TEM structure of trypanosomatid core editing complex (L-complex)
Authors:Li F, Ge P, Hui WH, Atanasov I, Rogersa K, Guo Q, Osatoa D, Falickd AM, Zhou ZH, Simpson L
Sample:L-Complex from L. major
Method:Single particle reconstruction (23 angstroms resolution)
Sample
Sample name: L-Complex from L. major
Oligomeric state: A single complex
Experimental molecular weight of the sample: 1.0
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinCore editing complex1.0complex of many subunitstrueLeishmania major
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.6 mg/mL20 mM Tris, pH 7.6, 60 mM KCl, 10 mM MgCl22% uranyl acetate for 2 mincontinuous carbon grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2 mm1600 nm2100 nm7000068200FIELD EMISSION GUNGENERIC CCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Single tilt room temperature holderSIDE ENTRY, EUCENTRIC°° eV300 K K K mrad400 e/Å201-AUG-2008
Processing
Protocol:projection matching
Software:EMAN
CTF correction:phase flip
Number of particles:12500
Number of class averages:879
Imposed symmetry:C1
Resolution by author:23 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
2615 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails