EMD-5577

Single-particle
5.0 Å
EMD-5577
3D View Gallery
Deposition: 28/01/2013
Map released: 24/04/2013
Last modified: 24/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5577
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j2w summary report (pdf.gz) Map and 3j2w FULL report (pdf.gz)
EMICSS annotations (xml)

Electron cryo-microscopy of Chikungunya virus

EMD-5577

Single-particle
5.0 Å
EMD-5577
3D View Gallery
Deposition: 28/01/2013
Map released: 24/04/2013
Last modified: 24/04/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.0
Details: PBS
Grid
Details: 400 mesh copper grid (1.2 um hole)
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 100 K
Instrument: FEI VITROBOT MARK I
Method: Blot for 2 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 59000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 250,000 times magnification, Electron beam tilt params: )
Image Recording [1]
Detector category: FILM
Detector model: GENERIC FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 6.35 µm
Number of real images: 1532
Average electron dose per image: 25 e/Å2
Image processing [1]
Final reconstruction
Resolution: 5.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 36236
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN, Frealign - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Icosahedral reconstruction of Chikungunya virus.
Details: ::::EMDATABANK.org::::EMD-5577::::
⬡ Geometry
X Y Z
Dimensions 648 648 324
Origin -324 -324 0
Spacing 648 648 324
Voxel size 1.11 Å 1.11 Å 1.11 Å
Contour list
Primary Level Source
True 1.1 AUTHOR