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Title:CS-tubulin Kinesin-13 Microtubule complex
Authors:Asenjo AB, Chatterjee C, Tan D, DePaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H
Sample:kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Method:Helical reconstruction (10.8 angstroms resolution)
Red flagLatest update:2013-04-10
Sample
Sample name: kinesin-13 KLP10A head domain in complex with CS-tubulin and a microtubule
Oligomeric state: Helical
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinKLP10AtrueDrosophila melanogasterQ960Z0KLP10A head domain
2proteinTubulin alpha-1A chainhelical multimerfalseBos taurusP81947Dimer with Tubulin beta-2B chain
3proteinTubulin beta-2B chainhelical multimerfalseBos taurusQ6B856Dimer with Tubulin alpha-1A chain
Experiment
Specimen state: Helical array
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Helical parameters:
HandPhi incrementAzimuthal (Z) increment
RIGHT HANDED168.08743°5.55267 Å
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KFEI VITROBOT MARK I ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 3200FSC300 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
JEOL°° eV K K K mrad e/Å201-MAY-2012
Gatan 626GATAN LIQUID NITROGEN°° eV K K K mrad e/Å201-MAY-2012
Processing
Software:Spider, IHRSR, CTFFIND3, Custom
CTF correction:Each particle
Resolution by author:10.8 Å
Resolution method:FSC calculated from two independently refined maps at 0.143 cut-off., gold-standard
Processing details:Custom single particle alignment method based on IHRSR.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixelCREO EVERSMART SUPREME
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails