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Title:Electron microscopy of Everglades virus
Authors:Sherman MB, Trujillo J, Leahy I, Razmus D, DeHate R, Lorcheim P, Czarneski MA, Zimmerman D, Newton JPM, Haddow AM, Weaver SC
Sample:Everglades virus
Method:Single particle reconstruction (10 angstroms resolution)
Red flagLatest update:2013-02-27
Sample
Sample name: Everglades virus
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusEverglades virusEverglades virus
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.41 mg/mL0.05 M Tris HCl, pH 7.4, 0.1 M NaCl, 0.001 M EDTAC-flat 1.2/1.3 holey film
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE60%90 KHOMEMADE PLUNGERblot for 4 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2200FS200 kVFLOOD BEAMBRIGHT FIELD2.0 mm300 nm2300 nm6000074800FIELD EMISSION GUNGATAN ULTRASCAN 4000 (4k x 4k) mmmanual correction at 150,000x

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Gatan 626 70 degree holderGATAN LIQUID NITROGEN°°omega eV88 K87 K90 K mrad20 e/Å209-FEB-2012
Processing
Protocol:angular reconstitution
Software:IMAGIC-5
CTF correction:micrograph
Number of particles:1000
Imposed symmetry:I
Resolution by author:10 Å
Resolution method:FSC 0.5
Processing details:Final maps were calculated from individual particles. Particles picked with EMAN2 boxer, reconstructed using IMAGIC-5.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
8015 μm/pixel16
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails