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Title:Electron microscopy of Everglades virus
Authors:Sherman MB, Trujillo J, Leahy I, Razmus D, DeHate R, Lorcheim P, Czarneski MA, Zimmerman D, Newton JPM, Haddow AM, Weaver SC
Sample:Everglades virus
Method:Single particle reconstruction (10 angstroms resolution)
Red flagLatest update:2013-02-27
Sample name: Everglades virus
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusEverglades virusEverglades virus
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.41 mg/mL0.05 M Tris HCl, pH 7.4, 0.1 M NaCl, 0.001 M EDTAC-flat 1.2/1.3 holey film
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE60%90 KHOMEMADE PLUNGERblot for 4 seconds before plunging ms
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2200FS200 kVFLOOD BEAMBRIGHT FIELD2.0 mm300 nm2300 nm6000074800FIELD EMISSION GUNGATAN ULTRASCAN 4000 (4k x 4k) mmmanual correction at 150,000x

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Gatan 626 70 degree holderGATAN LIQUID NITROGEN°°omega eV88 K87 K90 K mrad20 e/Å209-FEB-2012
Protocol:angular reconstitution
CTF correction:micrograph
Number of particles:1000
Imposed symmetry:I
Resolution by author:10 Å
Resolution method:FSC 0.5
Processing details:Final maps were calculated from individual particles. Particles picked with EMAN2 boxer, reconstructed using IMAGIC-5.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
8015 μm/pixel16
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails