top_cornerHomeEMD-5528  Contact EMDataBank 
Image unavailable
Title:Cryo-EM structure of the contracted bacteriophage T4 tail containing the collar and whiskers made of fibritin molecules.
Authors:Fokine A, Zhang Z, Kanamaru S, Bowman VD, Aksyuk AA, Arisaka F, Rao VB, Rossmann MG
Sample:bacteriophage T4
Method:Single particle reconstruction (25 angstroms resolution)
Red flagLatest update:2013-05-15
Sample
Sample name: bacteriophage T4
Components:
ID Type Name Exp. MW (MDa) Theo. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusEnterobacteria phage T4Enterobacteria phage T4
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
8.0 mg/mL50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 8 mM MgCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVSPOT SCANBRIGHT FIELD2 mm1500 nm3000 nm3800039190FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
GATAN LIQUID NITROGEN eV K K K mrad16 e/Å207-MAR-2007
Processing
Protocol:projection matching
Software:EMAN
CTF correction:phase flipping
Number of particles:2727
Imposed symmetry:C6
Resolution by author:25 Å
Resolution method:FSC 0.5
Processing details:The reconstruction of the contracted T4 tail which lacked the collar and whiskers (EMDB accession code 1086; Leiman et al., 2004, Cell 118, 419-429) was used as the initial model.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
977 μm/pixel8ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails